Journal: Journal of Cancer

Article Title: CXCL1 promotes cell migration in hepatocellular carcinoma by regulating the miR-30b-5p/ICAM-1 axis

doi: 10.7150/jca.95816

Figure Lengend Snippet: CXCL1 upregulates human HCC cell migration. (A-E) HCC cells were incubated with different concentrations of CXCL1 for 24 h; then, cell migration was assessed using the in vitro Transwell assay. (F) HCC cells were incubated with different concentrations of CXCL1 for 24 h; then, cell proliferation was assessed using the CCK8 assay. (G-H) Cells were treated with a CXCL1 shRNA; protein expression and cell migration were examined. Results are expressed as the mean ± SD of three independent experiments. * p < 0.05 as compared with controls.

Article Snippet: A human HCC tissue array was obtained from US Biomax (Derwood, Maryland, USA).

Techniques: Migration, Incubation, In Vitro, Transwell Assay, CCK-8 Assay, shRNA, Expressing

Journal: Journal of Cancer

Article Title: CXCL1 promotes cell migration in hepatocellular carcinoma by regulating the miR-30b-5p/ICAM-1 axis

doi: 10.7150/jca.95816

Figure Lengend Snippet: CXCL1 activates tumor cell migration via the ICAM-1 expression in human HCC cells. (A) Protein expression of ICAM-1 with concentration-depended CXCL1 treatment. (B) Cells were treated with ICAM-1 siRNA and then stimulated with CXCL1 (30 ng/mL); the cell migration was examined. (C) Cells were transfected with CXCL1 plasmid or shRNA, then CXCL1 expression was measured by Western blot. (D) Correlation analysis of CXCL1 and ICAM-1 expression using the GSE40367 database. Results are expressed as the mean ± SD of three independent experiments. * p < 0.05 as compared with controls; # p < 0.05 compared with the CXCL1-treated group.

Article Snippet: A human HCC tissue array was obtained from US Biomax (Derwood, Maryland, USA).

Techniques: Migration, Expressing, Concentration Assay, Transfection, Plasmid Preparation, shRNA, Western Blot

Journal: Cell reports

Article Title: Hyperpolarization-activated cyclic nucleotide-gated cation channel 3 promotes HCC development in a female-biased manner

doi: 10.1016/j.celrep.2023.113157

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Human HCC tissue array , TissueArray.Com LLC , Cat#LV1505a.

Techniques: Virus, Recombinant, Lysis, Extraction, SYBR Green Assay, DNA Extraction, Plasmid Preparation, CCK-8 Assay, Activity Assay, Enzyme-linked Immunosorbent Assay, Reporter Assay, RNA Sequencing Assay, Software

Journal: Gastroenterology Research and Practice

Article Title: Members of the Cyr61/CTGF/NOV Protein Family: Emerging Players in Hepatic Progenitor Cell Activation and Intrahepatic Cholangiocarcinoma

doi: 10.1155/2016/2313850

Figure Lengend Snippet: Localization of CCN2/CTGF protein in CK19 + human HPC that are surrounded by intratumoral α -SMA + myofibroblast cells. Immunofluorescent staining for CCN2/CTGF in combination with α -SMA (a) or CK19 (b) was carried out on human CCA sections. (d) The immunofluorescent staining revealed CCN2/CTGF localization in CK19 + human HCC tumor cells. (c and e) IH-CCA and HCC tumors in (b) and (d) were histologically analyzed by H&E staining. Scale bar: 30 μ m.

Article Snippet: Human intrahepatic CCA and HCC tissue arrays were purchased from US Biomax, Inc. (Rockville, MD).

Techniques: Staining

Journal: Journal of Cancer

Article Title: Metastasis-associated protein 2 regulates human hepatocellular carcinoma metastasis progression through modulating p38MAPK/MMP2 pathways

doi: 10.7150/jca.35626

Figure Lengend Snippet: Upregulation of MTA2 in HCC tissues associated with poor prognosis. (A) IHC analysis of MTA2 expression in normal liver tissues and tissues at different stages of HCC (stages 1-3). (B) Relative mRNA expression of MTA2 in normal liver tissues (N=49) and HCC tissues (N=374) from TCGA database analysis. (C) Kaplan-Meier survival curves regarding the low and high expression of MTA2 in HCC tissues from TCGA database analysis. (D.E) OS for HCC patients with a high or low expression of MTA2, as correlated with low- (grade-1) or high-grade (grade-2/3) HCC tissues according to TCGA database. *P < 0.05 and **P < 0.01 indicate a significant difference.

Article Snippet: A human HCC tissue array was purchased from US BioMax (LV1002).

Techniques: Expressing

Journal: Journal of Cancer

Article Title: Metastasis-associated protein 2 regulates human hepatocellular carcinoma metastasis progression through modulating p38MAPK/MMP2 pathways

doi: 10.7150/jca.35626

Figure Lengend Snippet: Effect of MTA2 knockdown on cell viability in human HCC cells. (A) Western blot shows MTA2 protein level in MTA2 knockdown SK-Hep-1 and Huh-7 cells. (B) Cell viability of the MTA2 knockdown cells was measured using MTT assay. (C) PI-stained cells show the analysis of cell cycle distribution rates using a flow cytometry assay. **P < 0.01 indicates a significant difference, compared with shLuc cells.

Article Snippet: A human HCC tissue array was purchased from US BioMax (LV1002).

Techniques: Western Blot, MTT Assay, Staining, Flow Cytometry

Journal: Journal of Cancer

Article Title: Metastasis-associated protein 2 regulates human hepatocellular carcinoma metastasis progression through modulating p38MAPK/MMP2 pathways

doi: 10.7150/jca.35626

Figure Lengend Snippet: MTA2 correlation with MMP2 and p38MAPK in HCC tissues. (A, B) GEPIA database revealed that MMP2 and p38MAPK levels are positively correlated with MTA2 expression in HCC tissues. P < 0.01 indicates a significant difference. (C) Graphical summary of MTA2 inhibits human HCC cell metastasis through the p38MAPK/MMP2 signaling pathways.

Article Snippet: A human HCC tissue array was purchased from US BioMax (LV1002).

Techniques: Expressing

Journal: Cancer cell

Article Title: Liver Cancer Initiation Requires p53 Inhibition by CD44-Enhanced Growth Factor Signaling

doi: 10.1016/j.ccell.2018.05.003

Figure Lengend Snippet: (A) Pan CD44 and CD44v6 IHC of vehicle (Veh) or DEN-challenged 5-month-old WT and 9-month-old Tak1ΔHep livers. (B) CD44 IHC of human normal liver and HCC. (C–D) CD44 mRNA expression in human normal liver and HCC specimens analyzed using Affymetrix Genome U133A 2.0 array (C) or Q-RT-PCR (D). (E–F) Edmonson tumor grading (E) and tumor differentiation (F) were categorized based on CD44 expression using patient samples shown in (D). For panels C–F, the results are expressed as Tukey’s boxplots where box indicates the 1st and 3rd quartiles, bar indicates median, whiskers indicate 1.5 interquartile range (IQR) and data beyond the end of the whiskers represent outliers. Mann-Whitney test was used to test the difference between two groups and Kruskal Wallis test for more than two groups. (G) Gross morphology of 9-month-old DEN-challenged WT and Cd44−/− livers. Tumor multiplicity, tumor size, and tumor incidence were determined. (H) Tumor multiplicity and tumor size in 9-month-old Tak1ΔHep and Cd44−/−;Tak1ΔHep livers. (I) 104 HcPC from 2-month-old Tak1ΔHep and Cd44−/−;Tak1ΔHep mice were transplanted into MUP-uPA mice. Tumor multiplicity was assessed 6 months later (n ≥ 6 mice/group). (J) 104 HcPC from 5-month-old DEN-treated WT mice were transplanted into either MUP-uPA or MUP-uPA;Cd44−/− hosts and tumor multiplicity was assessed 6 months later (n ≥ 3 mice/group). (K) Cd44F/F and Cd44ΔHep males were DEN-challenged and tumor multiplicity and size were determined 9 months later. All bar graphs in panels G–K represent the mean ± SEM. See also Figure S1.

Article Snippet: Human HCC tissue array , US Biomax, Inc. , Cat# LV1501.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, MANN-WHITNEY

Journal: Cancer cell

Article Title: Liver Cancer Initiation Requires p53 Inhibition by CD44-Enhanced Growth Factor Signaling

doi: 10.1016/j.ccell.2018.05.003

Figure Lengend Snippet: (A–C) WT and Cd44−/− males (8–12-week-old) were DEN-challenged (100 mg/kg), their livers were collected when indicated and IHC-analyzed for Mdm2 (A), phospho-S473 Akt (B) and phospho-S166 Mdm2 (C) (n ≥ 6 different fields from 3 different mice for each time point; mean ± SEM). (D) WT mice were treated with Veh or MK2206 (100 mg/kg/day) starting one day prior to DEN challenge (100 mg/kg). Livers were collected 48 hr later and IHC analyzed with the indicated antibodies (n ≥ 3 mice/group). (E) Human HCC tissue array was IHC-analyzed for CD44 and phospho-S166 MDM2. (C = central vein). See also Figure S5.

Article Snippet: Human HCC tissue array , US Biomax, Inc. , Cat# LV1501.

Techniques:

Journal: Cancer cell

Article Title: Liver Cancer Initiation Requires p53 Inhibition by CD44-Enhanced Growth Factor Signaling

doi: 10.1016/j.ccell.2018.05.003

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Human HCC tissue array , US Biomax, Inc. , Cat# LV1501.

Techniques: Recombinant, Blocking Assay, In Situ, TUNEL Assay, Plasmid Preparation, Amplification, Staining, Labeling, In Situ Hybridization, RNAscope 2.5 HD Assay, SYBR Green Assay, Negative Control, Software